Microscopie

Acknowledging the facility in your works:

One of our funder the France-BioImaging National Infrastructure does request to be acknowledged in your publication (https://france-bioimaging.org/about/acknowledgments/  )

All the users (and thus authors) are required to acknowledge France BioImaging when they use one of the FBI core facilities or technologies and methodologies developed through FBI funding. Papers from FBI funded Teams or scientific collaboration within or with FBI members are also concerned. The mandatory term is:

“The National Infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04)”

Depending on the context, we suggest two model sentences:

  • For the publications of scientists using one of the France-BioImaging Imaging Facilities:  “We acknowledge the IBMP Cell Imaging Facility, member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04)”
  • For the publications by FBI members (technological development or scientific projects and collaborations) the sentence must appear in tributes as follows: “We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04)

 

News

Voici le webinaire d’introduction des extensions Inkscape donné dans le cadre du RT-MFM:

I usually share news tools I develop here:

Personnel

Mathieu Erhardt et Jérôme Mutterer

Rules for usage of microscopes

Follow official rules described in the following statement :

CNRS statement.

Microscopy professional networks suggest following some specific rules for usage of microscopes in COVID times. Here a copy of what I posted on microscopy room doors. (Poster created by Marion Decossas)

Facility access

Dear all, we kindly remind everyone that you need to get trained prior to using microscopy devices in the IBMP.
If you need to use a device and you didn’t get such a training or introduction, please inquire.
Access to microscopes is strictly restricted to staff trained by their supervisor, facility staff, or other appropriate advanced user.
If you still have doubts, get in touch.

Mathieu & Jerome.

Recommended educational resources

Je vous recommande vivement une série de cours, réalisée par François Michel (Inmed, Marseille), qui traite des fondamentaux de la microscopie optique.
 

Current data management plan

All users are requested to get their data with them at the end of their imaging session. Most systems are driven by PCs connected to our intranet, so you can connect to your group’s shared folder. Do not leave data on acquisition PCs, as these workstations are shared access, so someone else  might alter your files by mistake. Also, computers sometimes fail without prior notice. 

Also, please read a suggested workflow for microscopy data.

Booking our systems

Link to GRR microscope booking system

Devices

  • Zeiss LSM700 confocal (FAQ)
    • Variable beam splitter, 2 fluorescence channels+transmitted light
    • 405, 488, 555, 633 nm lasers
  • Zeiss LSM 780 confocal (FAQ)
    • Channel mode: 3 adjustable fluorescence channels, spectral mode: 32 channels
    • 405, 488, 561, 633 nm lasers
  • Leica SP8 confocal (Mon-Wed : all users ; Thu-Sun : group P. Genschik)
    • Spectral mode using AOBS, Standard + HyD detectors
    • performances similar to LSM780
  • Zeiss AxioImager Z1
    • Motorized widefield fluorescence microscope, high sensitivity BW camera, routine color camera, optional apotome mode
    • Available objectives :
      • dry : 10×0.45, 20×0.8, 40×0.95
      • oil  : 63×1.4,  100×1.4
  • Nikon E800
    • Widefield fluorescence microscope, routine color camera
    • lesser sensitive device, can be used for screening
  • Nikon TE 2000
  • Leica DMBR
    • Brightfield microscope for histology. Color camera.
  • Leica Macroscope
    • Macroscope with flexible working distance. Color camera shared with Zeiss stereomicroscope in the same room.
  • Zeiss Axiozoom (important information)
    • Motorized fluorescence macroscope. High sensitivity BW camera. Fluorescence only, no Brightfield mode.
  • Zeiss StereoMicroscope Stemi 305
  • Nikon StereoMicroscope SMZ 800
  • Leica StereoMicroscope (4th floor)
  • Serial Block Face Scanning Electron Microscope
    • Scanning electron microscope equipped with a in-chamber Gatan 3ViewXP2 ultra-microtome
  • Tabletop Scanning Electron Microscope Hitachi TM 1000
    • Routine Scanning Electron Microscope. Works in low-vaccum mode, no special sample preparation needed