Microscopy

Staff

Mathieu Erhardt and  Jérome Mutterer

Facility access

Dear all, I kindly remind everyone that you need to get trained prior to using microscopy devices in the IBMP.
If you need to use a device and you didn’t get such a training or introduction, please inquire.
Access to microscopes is strictly restricted to staff trained by their supervisor, facility staff, or other appropriate advanced user.
If you still have doubts, get in touch.

Recommended educational resources

By François Michel (Inmed, Marseille), fundamentals of optical microscopy

Current data management plan

All users are requested to get their data with them at the end of their imaging session. Most systems are driven by PCs connected to our intranet, so you can connect to your group’s shared folder. Do not leave data on acquisition PCs, as these workstations are shared access, so someone else (not as smart as you are) might alter your files by mistake. Also, computers sometimes fail without prior notice.

Also, please read a suggested workflow for microscopy data.

Booking our systems

Link to GRR microscope booking system

Devices

  • Zeiss LSM700 confocal (FAQ)
    • Variable beam splitter, 2 fluorescence channels+transmitted light
    • 405, 488, 555, 633 nm lasers
  • Zeiss LSM 780 confocal (FAQ)
    • Channel mode: 3 adjustable fluorescence channels, spectral mode: 32 channels
    • 405, 488, 561, 633 nm lasers
    • Currently Out of Order, history as follows:
      • 11/07 the Argon laser fails
      • 11/09 an new laser has been ordered
      • 11/16 new laser received
      • 11/30 Zeiss services the laser. Everything works
      • 12/04 Power supply failure
      • 12/04 New power supply ordered
      • 12/12 New Power supply received, laser still doesn’t start
      • 12/13 New power supply + laser combo ordered
      • 12/13 Info from Zeiss: earliest possible service on 2018/01/10
      • 2018/01/09 Argon laser and power supply replaced
      • 01/09 remaining issue: bad rotation of the objective turret
  • Leica SP8 confocal (group P. Genschik)
    • Spectral mode using AOBS, Standard + HyD detectors
  • Zeiss AxioImager Z1
    • Motorized widefield fluorescence microscope, high sensitivity BW camera, routine color camera, optional apotome mode
    • Available objectives :
      • dry : 10×0.45, 20×0.8, 40×0.95
      • oil  : 63×1.4,  100×1.4
  • Nikon E800
    • Widefield fluorescence microscope, routine color camera
  • Nikon TE 2000
  • Leica DMBR
    • Brightfield microscope for histology. Color camera.
  • Leica Macroscop
    • Macroscope with flexible working distance. Color camera shared with Zeiss stereomicroscope in the same room.
  • Zeiss Axiozoom (important information)
    • Motorized fluorescence macroscope. High sensitivity BW camera. Fluorescence only, no Brightfield mode.
  • Nikon StereoMicroscope
  • Leica StereoMicroscope
  • Serial Block Face Scanning Electron Microscope
    • Scanning electron microscope equipped with a in-chamber Gatan ultra-microtome.
  • Tabletop Scanning Electron Microscope
    • Routine Scanning Electron Microscope. Works in low-vaccum mode, no special sample preparation needed