p3P- E. coli expression strains

 

Name Parental strain Resistance Feature Competent Origin
BL21 (DE3) BL21 Classic highly productive strain   Novagen
Bl21 (DE3) pLysS BL21 Cm + LysY =repression of leaky expression   Novagen
RosettaII (DE3) pLysS BL21 Cm 7 rare codons tRNAs for eukaryotic proteins   Novagen
Rosetta-gami 2(DE3) pLysS K-12

Tet + Str + Cam

7 rare codons tRNAs for eukaryotic proteins+ enhances disulfide bond formation   Novagen
Rosetta-gami B(DE3) pLysS BL21

Kan + Tet + Cam

6 rare codons tRNAs for eukaryotic proteins+ enhances disulfide bond formation+ LacZY mutant (Tuner)   Novagen
Rosetta Blue (DE3) pLysS K12

Tet + Cam

6 rare codons tRNAs for eukaryotic proteins+recA- , endA- , lacI q ; high transformation efficiency

  Novagen
Origami 2(DE3) pLysS K12

Tet + Str + Cam

trxB/gor mutantpromote disulfide bond formation   Novagen
Origami B(DE3) pLysS BL21

Kan + Tet + Cam

promote disulfide bond formation; LacZY mutant (Tuner)   Novagen
SoluBL21 (DE3)   selected evolution for soluble protein expression   GenLantis
SoluBL21 (DE3) pLysS   Cm selected evolution for soluble protein expression   GenLantis
Arctic Express Bl21 Gt expression of low Temp adapted chaperone Cpn 60/10   Agilent
SHuffle T7 Express   SpcR promote disulfide bond formation   NEB
BL21(DE3) pG-KJE8   Cm dnaK-dnaJ-grpE-groES-groEL chaperones   Takara
BL21(DE3) pGro7   Cm groES-groEL chaperones   Takara
BL21(DE3) pKJE7   Cm dnaK-dnaJ-grpE chaperones   Takara
BL21(DE3) pG-Tf2   Cm groES-groEL-Tig chaperones   Takara
BL21(DE3) pTf16   Cm tig chaperone   Takara
BL21(DE3) pBB530/535   Cm/Spc GrpE DnaK DnaJ   DeMarco Lab
BL21(DE3) pBB540/535   Cm/Spc GrpE ClpB DnaK DnaJ   DeMarco Lab
BL21 (DE3)pBB528/541   Cm/Spc GroESL   DeMarco Lab
BL21 (DE3)pBB540/542   Cm/Spc GrpE ClpB DnaK DnaJ GroESL (high)   DeMarco Lab
BL21 (DE3)pBB540/550   Cm/Spc GrpE ClpB DnaK DnaJ GroESL (low)   DeMarco Lab
BL21 (DE3)pBB528/572   Cm/Amp ibpA/B   DeMarco Lab
BL21 (DE3)pBB528/541/572   Cm/Spc/Amp ibpA/B GroESL   DeMarco Lab
BL21 (DE3)pBB540/550/572   Cm/Spc/Amp GrpE ClpB DnaK DnaJ GroESL ibpA/B   DeMarco Lab
Lemo 21   Cm tunable Expression level with Rhamnose induced LysY   NEB
HMS174     helpfull for toxic proteins   Novagen

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Resistance: in bold= selection must be performed in order to maintain plasmids