p3P-Equipments

Cultures:

Refrigerated rotary incubator Innova 44R

  •  2-L flasks (max 12);250ml flasks
  • Booking through eBooking

Refrigerated rotary incubator Innova 42R

  •  2-L flasks (max 6);  250ml flasks; 24 deep wells Plate
  • Booking through eBooking

Centrifugation:

Sorvall XTR centrifuge

  • Fixed angle FiberLite 8x50ml (Falcon tubes; 26 000g max)
  • Swing out 4x 500 ml buckets with insert for 15ml and 50ml Falcon tubes (4000g max)
  •  Swing out 4x microplate holders (4000g max)
  • No booking; enquiry to P3P personnel

Cell Lysis:

Sonicator Vibracell (room 512)

  • 13mm probe (samples >10ml-100ml)
  • 5mm probe (samples <5ml; max amplitude 40%)
  • 4×5 mm probe (samples <5ml; max amplitude 40%)
  • Booking through eBooking

French press (room 534)

  • 30ml cell; 10ml cell (in cold room 537)

Microfluidizer LM20-30 (room 536)

  • 10ml-1L continuous processing
  • Booking through eBooking

Chromatography:

Detailed documentation: see here

Akta Purifier 10

  • For general chromatography (loop and sample pump injection); analytical SEC;
  • Booking through eBooking; Requires training by P3P personnel

Aktra Pure 25

  • General chromatography; loop-valve; column-valve; buffer A -valve; Bi-dimension chromatography;
  • Not appropriate for small-scale analytical or high resolution SEC due to extended tubing length
  • Booking through eBooking; Requires training by P3P personnel

Electrophoresis and gel imaging:

CBS vertical electrophoresis system (30cm wide gels)

  • combs 31, 50, 102 teeth

Ettan DALTsix electrophoresis system

Protean IEF Cell (electrofocusing for 2D-gel strips)

Ettan DIGE Imager (room 606)

  • Fluorescence scanner

Fusion FX documentation system (room 532 and 606)

  • On both system:Luminescence; epi white light; transmitted UV; transmitted white light;
  • Only on system room 606: fluorescence Fusion FX7 manual)