p3P-Equipments

Cultures:

Refrigerated rotary incubator Innova 44R

  •  2-L flasks (max 12);250ml flasks
  • Booking through GRR (priority is given to cultures for protein expression)

Refrigerated rotary incubator Innova 42R

  •  2-L flasks (max 6);  250ml flasks; 24 deep wells Plate
  • Booking through GRR (priority is given to cultures for protein expression)

Centrifugation:

Sorvall XTR centrifuge

  • Fixed angle FiberLite 8x50ml (Falcon tubes; 26 000g)
  • Swing out 4x 500 ml buckets with insert for 15ml and 50ml Falcon tubes (4000g)
  •  Swing out 4x microplate holders (4000g)
  • No booking; enquiry to P3P personnel

Cell Lysis:

Sonicator Vibracell (room 512) (Ecoli lysis by sonication procedure)

  • 5mm (samples> 0,5-10ml; max amplitude 40%)  and 13mm probes (samples >10ml-100ml; max amplitude 100%)
  • Booking through GRR

French press (room 534) (french press operation proecedure)

  • 30ml cell; 10ml cell (in cold room 537)

LM20-30 (room 536) microfluidizer (LM20 operation procedure)

  • suitable for E. coli lysis (1000bars) and yeast( 1700bars)
  • 10ml-1L continuous processing
  • Booking through GRR

Chromatography:

Akta Purifier 10 (manuals)

  • For general chromatography (loop and sample pump injection); analytical SEC;
  • Booking through GRR; Requires training by P3P personnel
  • Unicorn 7 software (manuals)

Aktra Pure 25 (manuals)

  • General chromatography; loop-valve; column-valve; buffer A -valve; Bi-dimension chromatography;
  • Not appropriate for small-scale analytical or high resolution SEC due to extended tubing length
  • Booking through GRR; Requires training by P3P personnel
  • Unicorn 7 software (manuals)

Dialysis and Buffer Exchange:

Amicon Ultrafiltration units: (single use)

  • MWCO 3/30/100kDa
  • Vol 0,5/4/15ml

Micro-dialysis button Hampton Research: (reusable, to be used with dialysis membrane of desired cut-off)

  • sample volume: 5/10/15/20/50/100/200/350ul
  • To be returned to the plateforme after use
  • dialysis membrane available: CelluSep 6000-8000 MWCO; SpectraPore 1000 MWCO

Desalting chromatography columns: (To be used on Akta FPLC system)

  • HiTrap Desalting 5ml (1,5ml sample)
  • HiPrep Desalting 26/10 (15ml sample)

Electrophoresis and gel imaging:

CBS vertical chromatography system

  • combs 31, 50, 102 teeth

Biorad Tetrad cell

Ettan DALTsix electrophoresis system

Protean IEF Cell

Fusion FX documentation system (room 532 and 606)