P3P- GWA vectors and tags

Gateway bacterial Expression vectors:

Vector Name N-ter Tag C-ter Tag
pGWA  no optional 6xHis
pHGWA  6xHis optional 6xHis
pGGWA  GST optional 6xHis
pMGWA  MBP optional 6xHis
pNGWA  NusA optional 6xHis
pXGWA Thioredoxin optional 6xHis
pHGGWA  6xHis-GST optional 6xHis
pHMGWA  6xHis-MBP optional 6xHis
pHNGWA  6xHis-NusA optional 6xHis
pHXGWA  6xHis-TRX optional 6xHis
pHSGWA   6xHis-StrepII  optional 6xHis
 pHSGGWA  6xHis-StrepII-GST  optional 6xHis
 pHSMGWA  6xHis-StrepII-MBP  optional 6xHis
 pHSNGWA  6xHis-StrepII-NusA  optional 6xHis
 pHSXGWA  6xHis-StrepII-TRX  optional 6xHis

Provides the DNA and Protein sequences of all the tags available in the pGWA destination vectors serie, from the translation start to the fusion with the GOI, including the spacer and recombination site.

Tags sequences

Original publication describing the gateway vector serie