Gene Expression Analysis
The goal of the Gene Expression Analysis Platform (AEG) is to provide to public and private research teams some tools and competences for the realization of their research projects related to genomics, transcriptomics and epigenetics .
This technology platform is shared between 3 research units from Strasbourg, IBMC, GMGM and IBMP.
The platform has instruments for the realization of sequencing projects (Oxford Nanopore, NGS-Illumina and Sanger), genotyping (HRM) , gene expression analysis (qPCR) and digital PCR (ddPCR)
Equipments
- Oxford Nanopore Sequencing: PromethION P2 and 2 MinION with Flongle Adapter
- Illumina NGS sequencing: MiSeq
- Digital PCR: Stilla Naica System
- Genes expression analysis by qPCR and HRM genotyping: Roche LC480-II
- Quality control of NGS sequencing samples and libraries: Bioanalyzer 2100 (Agilent); Qubit (ThermoFisher Scientific)
Contact the platform?
To submit a project to the AEG platform, benefit from training or support in carrying out your analyses, contact us by email
Access to platform instruments
Some instruments on the platform are freely accessible. This access is governed by a charter for the use of IBMP platforms and technical facilities.
Expertises
Sanger sequencing (AppliedBiosystems)
Full length plasmid sequencing
NGS short reads sequencing (Illumina)
NGS long reads sequencing (Oxford Nanopore)
Realtime quantitative PCR (Roche)
High throughput HRM genotyping (Roche)
Digital PCR (Stilla)
Recognition and promotion
The platform must be mentioned in the acknowledgments of publications. If the degree of involvement in the work justifies it, the platform staff should be included in the authors of the publications. A copy of the articles will be sent to the platform manager after publication.