P3P- E. coli protocols 22.06.2018 Chimio-competent CaCl2 protocol Chimio-competent Inoue protocol Usual Bacterial media recipes E.coli protein expression (IPTG) E.coli Lysis with Microfluidizer E.coli lysis by sonication Small scale E.coli expression and Ni-NTA purification-low throughput version